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KMID : 0362320010260040285
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Journal of Korean Academy of Operative Dentistry
2001 Volume.26 No. 4 p.285 ~ p.295
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THE INHIBITORY EFFECT OF TAURINE AND ALENDRONATE ON THE OSTEOCLAST DIFFERENTIATION MEDIATED BY SONICATED EXTRACTS OF PORPHYROMONAS GINGIVALIS IN VITRO
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Park Ju-Hyun
Kum Kee-Yeon
Lee Jung-Hyun
Yu Yoon-Jeong
Lee Seung-Jong
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Abstract
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The objective of this study was to investigate the inhibitory effect of taurine and alendronate on the osteoclast differentiation. Osteoblasts and bone marrow cells from 1-2 day old mouse were co-cultured in 10% fetal bovine serum - minimal essential media (FBS-MEM). Osteoclast differentiation was induced by adding the sonicated extracts of Porphyromonas gingivalis (P.gingivalis). Osteoclasts were identified using tartrate resistant acid phosphotase staining (TRAP). Alendronate of 10-7, 10-6, 10-5M and taurine of 500, 1000, 1500μg/ml were added respectively. The cytotoxic effects of alendronate and taurine were examined using MTT(3-(4,5-dimethylthiazol -2-yl-2,5 -diphenyltetrazo- lium bromide) method. After culturing with the sonicated extracts of P. gingivalis, the amounts of IL-6 in the culture supernatants were measured and compared using the ELISA method. The results were as follows:
1. Osteoclasts were differentiated at the concentration of 0.01??0.1μg/ml sonicated extracts of P.gingivalis. (P<0.05)
2. Alendronate inhibited osteoclasts differentiation at the concentration of 10-5 M when the concentration of sonicated extracts of P.gingivalis was 0.01μg/ml.
3. Taurine inhibited osteoclasts differentiation at the concentration of 1500μg/ml when the concentration of sonicated extracts of P.gingivalis was 0.01μg/ml.
4. In cytotoxic test (MTT test), no cytotoxic effect was evident in all concentrations of alendronate and taurine.
5. Taurine(10-5M) and alendronate(1500μg/ml) did not change the amounts of IL-6 induced by sonicated extracts of P. gingivalis significantly.
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KEYWORD
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Alendronate , Taurine , P.gingivalis , Osteoclast , Replacement root resorption
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